Coding

Part:BBa_K1189030:Design

Designed by: Amanda Zucoloto   Group: iGEM13_Calgary   (2013-09-17)

TALE-B with a his6 tag linked to a K coil under an inducible lacI promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2654
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design notes

The TALE sequence was codon optimzed, a Kozak sequence eliminated, generated through a commericial synthesis company.

Source

The TALE sequence was codon optimzed, a Kozak sequence eliminated, generated through a commericial synthesis company.

References

  • Litowski, J. R., & Hodges, R. S. (2002). Designing heterodimeric two-stranded alpha-helical coiled-coils. Effects of hydrophobicity and alpha-helical propensity on protein folding, stability, and specificity. The Journal of Biological Chemistry, 277(40), 37272–9. doi:10.1074/jbc.M204257200